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mem neaa  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mem neaa
    Mem Neaa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mem-neaa/2+MERCAPTOETHANOL/pm41941846-91-24-26
    Average 99 stars, based on 1 article reviews
    mem neaa - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Detection of low pre-existing humoral immunity against influenza virus H5N1 clade 2.3.4.4b in unexposed individuals
    Article Snippet: MDCK-SIAT1 (Merck, cat. no. 5071502) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma-Aldrich) containing 10% FBS (Sigma Aldrich), 1% Penicillin-Streptomycin (PS, Gibco), 1 mM L-Glutamine (Gibco) and 1% MEM-NEAA (Gibco).

    Article Title: Impaired Myelination in Multiple Sclerosis Organoids: p21 Links Oligodendrocyte Dysfunction to Disease Subtype
    Article Snippet: On day 3 to totality of the culture media was removed and replaced with a differentiation media consisting of 1:1 DMEM-F12 and Neurobasal medium (Gibco), with addition of 0.5% N2 supplement (Life Technologies), 0.5% ml MEM-NEAA (Gibco), 1% Glutamax (Gibco), 1% B27 supplement without vitamin A (Life Technologies), 0.1 μM of 2-mercaptoethanol (Millipore), 2.6 μg/ml insulin (Sigma Aldrich) supplemented with 10μM of SB431542 (STEMCELL), 2 μM of CHIR99021 (STEMCELL) and 0.5 μM of LDN-193189 (STEMCELL).

    Article Title: Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging.
    Article Snippet: Briefly, for hCS, 9000 iPSCs were first treated with 20 μM of ROCK inhibitor (S1049, Selleckchem) in neural induction medium containing 20% knockout serum replacement (10828028, Gibco), 1% GlutaMax (35050061, Gibco), 1% MEM-NEAA (11140035, Gibco), 0.1 mM of 2-mercaptoethanol (21985023, Gibco), and 1% penicillin/streptomycin (PS-B, Capricorn) in DMEM/F12 media.

    Article Title: Altered Protein Phosphorylation in a Novel Midbrain Organoid Model for Bipolar Disorder
    Article Snippet: Starting at day 14, media changes took place every other day using Midbrain 4th media Neurobasal medium (Gibco/Fisher Scientific), 1X B27 Supplement (Thermo Fisher), 1X MEM-NEAA (Gibco/Fisher Scientific), 1X GlutaMax (Thermo Fisher), 1X Penicillin/Streptomycin (Gibco/Fisher Scientific), 200 μM Ascorbic Acid (Sigma Aldrich), 500 μM cAMP (Sigma Aldrich), 20 ng/mL BDNF (Peprotech/Thermo Fisher), 20 ng/mL GDNF (Peprotech/Thermo Fisher), and 1 ng/mL TGF beta (Stemcell Technologies).

    Article Title: Maternal phthalates exposure promotes neural stem cell differentiation into phagocytic astrocytes and synapse engulfment via IRE1α/XBP1s pathway.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pHrodo Thermo Fisher Scientific Cat#P36600 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat#13778150 mTeSRTM Plus medium STEMCELL Technologies Cat#100-0276 Matrigel Corning Cat#356231 ReleSR STEMCELL Cat#100-0484 Y-27632 STEMCELL Technologies Cat#72308 knockout serum replacer Thermo Fisher Scientific Cat#17504044 MEM-NEAA Thermo Fisher Scientific Cat#11140050 dorsomorphine Tocris Cat#3093/10 A83-01 Tocris Cat#2939/10 b-mercaptoethanol Gibco Cat#21985023 N2 supplement Thermo Fisher Scientific Cat#17502048 Heparin Meilunbio Cat#MB1908 SB431542 Abcame Cat#ab120163 LDN193189 Sigma Cat#SML0559 Insulin Beyotime Cat#P3376-400IU MKC8866 Fosun Orinove N/A MBP MACKLIN Cat#131-70-4 Tamoxifen MedChemExpress Cat# HY-13757A Critical commercial assays Cell Count Kit-8 Vazyme Cat#A311-01 DAB kit Absin Bioscience Cat#ABS957 FD Rapid GolgiStainTM Kit FD NeuroTechnologies Cat#PK-401 Universal cDNA synthesis kit Vazyme Cat#R233-01 SYBR Green master mix Vazyme Cat#Q311-02 TRIzol reagent box Thermo Fisher Scientific Cat#15596018 Firefly & Renilla Luciferase Reporter Assay Kit Meilunbio Cat#MA0518 Fluo-3a.m.

    Article Title: Mechanical impact on neural stem cell lineage decisions in human brain organoids
    Article Snippet: ODM consisted of a 1:1 mix of DMEM/F12 + GlutaMAX-I (Thermo Fisher) and Neurobasal medium (Thermo Fisher), 0,5% N2 supplement (Thermo Fisher), 0.1 mM MEM-NEAA (Thermo Fisher), 100 U/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher), 1% B27 +/- vitamin A supplement (Thermo Fisher), 0.025% insulin (Sigma-Aldrich), 0.035% 2-mercaptoethanol (Sigma-Aldrich).

    Article Title: A stroke organoids-multiomics platform to study injury mechanism and drug response
    Article Snippet: Stroke is one of the top causes of death and disability worldwide, and its pathogenesis and mechanism have not been fully elucidated.. Several agents have shown protective effects against stroke in animal models; however, few studies have shown obvious effects in clinical practice.. This might be due to differences in brain structure and physiological function between humans and rodents.

    Article Title: Impaired Myelination in Multiple Sclerosis Organoids: p21 Links Oligodendrocyte Dysfunction to Disease Subtype
    Article Snippet: After neuroepithelium emergence (typically at ∼ day 9-10), embryoid bodies were embedded in 15 μL Matrigel droplets and cultured in 6 well plates containing c-organoid differentiation medium consisting of 1:1 DMEM-F12 and Neurobasal medium (Gibco), with addition of 0.5% N2 supplement (Life Technologies), 0.5% ml MEM-NEAA (Gibco), 1% Glutamax (Gibco), 1% B27 supplement without vitamin A (Life Technologies), 0.1 μM of 2-mercaptoethanol (Millipore), 2.6 μg/ml insulin (Sigma Aldrich) in static culture for 4 days.

    Knock-Out:

    Article Title: Detection of low pre-existing humoral immunity against influenza virus H5N1 clade 2.3.4.4b in unexposed individuals
    Article Snippet: MDCK-SIAT1 (Merck, cat. no. 5071502) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma-Aldrich) containing 10% FBS (Sigma Aldrich), 1% Penicillin-Streptomycin (PS, Gibco), 1 mM L-Glutamine (Gibco) and 1% MEM-NEAA (Gibco).

    Article Title: Impaired Myelination in Multiple Sclerosis Organoids: p21 Links Oligodendrocyte Dysfunction to Disease Subtype
    Article Snippet: On day 3 to totality of the culture media was removed and replaced with a differentiation media consisting of 1:1 DMEM-F12 and Neurobasal medium (Gibco), with addition of 0.5% N2 supplement (Life Technologies), 0.5% ml MEM-NEAA (Gibco), 1% Glutamax (Gibco), 1% B27 supplement without vitamin A (Life Technologies), 0.1 μM of 2-mercaptoethanol (Millipore), 2.6 μg/ml insulin (Sigma Aldrich) supplemented with 10μM of SB431542 (STEMCELL), 2 μM of CHIR99021 (STEMCELL) and 0.5 μM of LDN-193189 (STEMCELL).

    Article Title: Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging.
    Article Snippet: Briefly, for hCS, 9000 iPSCs were first treated with 20 μM of ROCK inhibitor (S1049, Selleckchem) in neural induction medium containing 20% knockout serum replacement (10828028, Gibco), 1% GlutaMax (35050061, Gibco), 1% MEM-NEAA (11140035, Gibco), 0.1 mM of 2-mercaptoethanol (21985023, Gibco), and 1% penicillin/streptomycin (PS-B, Capricorn) in DMEM/F12 media.

    Article Title: Altered Protein Phosphorylation in a Novel Midbrain Organoid Model for Bipolar Disorder
    Article Snippet: Starting at day 14, media changes took place every other day using Midbrain 4th media Neurobasal medium (Gibco/Fisher Scientific), 1X B27 Supplement (Thermo Fisher), 1X MEM-NEAA (Gibco/Fisher Scientific), 1X GlutaMax (Thermo Fisher), 1X Penicillin/Streptomycin (Gibco/Fisher Scientific), 200 μM Ascorbic Acid (Sigma Aldrich), 500 μM cAMP (Sigma Aldrich), 20 ng/mL BDNF (Peprotech/Thermo Fisher), 20 ng/mL GDNF (Peprotech/Thermo Fisher), and 1 ng/mL TGF beta (Stemcell Technologies).

    Article Title: Maternal phthalates exposure promotes neural stem cell differentiation into phagocytic astrocytes and synapse engulfment via IRE1α/XBP1s pathway.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pHrodo Thermo Fisher Scientific Cat#P36600 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat#13778150 mTeSRTM Plus medium STEMCELL Technologies Cat#100-0276 Matrigel Corning Cat#356231 ReleSR STEMCELL Cat#100-0484 Y-27632 STEMCELL Technologies Cat#72308 knockout serum replacer Thermo Fisher Scientific Cat#17504044 MEM-NEAA Thermo Fisher Scientific Cat#11140050 dorsomorphine Tocris Cat#3093/10 A83-01 Tocris Cat#2939/10 b-mercaptoethanol Gibco Cat#21985023 N2 supplement Thermo Fisher Scientific Cat#17502048 Heparin Meilunbio Cat#MB1908 SB431542 Abcame Cat#ab120163 LDN193189 Sigma Cat#SML0559 Insulin Beyotime Cat#P3376-400IU MKC8866 Fosun Orinove N/A MBP MACKLIN Cat#131-70-4 Tamoxifen MedChemExpress Cat# HY-13757A Critical commercial assays Cell Count Kit-8 Vazyme Cat#A311-01 DAB kit Absin Bioscience Cat#ABS957 FD Rapid GolgiStainTM Kit FD NeuroTechnologies Cat#PK-401 Universal cDNA synthesis kit Vazyme Cat#R233-01 SYBR Green master mix Vazyme Cat#Q311-02 TRIzol reagent box Thermo Fisher Scientific Cat#15596018 Firefly & Renilla Luciferase Reporter Assay Kit Meilunbio Cat#MA0518 Fluo-3a.m.

    Article Title: Mechanical impact on neural stem cell lineage decisions in human brain organoids
    Article Snippet: ODM consisted of a 1:1 mix of DMEM/F12 + GlutaMAX-I (Thermo Fisher) and Neurobasal medium (Thermo Fisher), 0,5% N2 supplement (Thermo Fisher), 0.1 mM MEM-NEAA (Thermo Fisher), 100 U/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher), 1% B27 +/- vitamin A supplement (Thermo Fisher), 0.025% insulin (Sigma-Aldrich), 0.035% 2-mercaptoethanol (Sigma-Aldrich).

    Article Title: A stroke organoids-multiomics platform to study injury mechanism and drug response
    Article Snippet: Stroke is one of the top causes of death and disability worldwide, and its pathogenesis and mechanism have not been fully elucidated.. Several agents have shown protective effects against stroke in animal models; however, few studies have shown obvious effects in clinical practice.. This might be due to differences in brain structure and physiological function between humans and rodents.

    Article Title: Impaired Myelination in Multiple Sclerosis Organoids: p21 Links Oligodendrocyte Dysfunction to Disease Subtype
    Article Snippet: After neuroepithelium emergence (typically at ∼ day 9-10), embryoid bodies were embedded in 15 μL Matrigel droplets and cultured in 6 well plates containing c-organoid differentiation medium consisting of 1:1 DMEM-F12 and Neurobasal medium (Gibco), with addition of 0.5% N2 supplement (Life Technologies), 0.5% ml MEM-NEAA (Gibco), 1% Glutamax (Gibco), 1% B27 supplement without vitamin A (Life Technologies), 0.1 μM of 2-mercaptoethanol (Millipore), 2.6 μg/ml insulin (Sigma Aldrich) in static culture for 4 days.

    Cell Counting:

    Article Title: Detection of low pre-existing humoral immunity against influenza virus H5N1 clade 2.3.4.4b in unexposed individuals
    Article Snippet: MDCK-SIAT1 (Merck, cat. no. 5071502) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma-Aldrich) containing 10% FBS (Sigma Aldrich), 1% Penicillin-Streptomycin (PS, Gibco), 1 mM L-Glutamine (Gibco) and 1% MEM-NEAA (Gibco).

    Article Title: Impaired Myelination in Multiple Sclerosis Organoids: p21 Links Oligodendrocyte Dysfunction to Disease Subtype
    Article Snippet: On day 3 to totality of the culture media was removed and replaced with a differentiation media consisting of 1:1 DMEM-F12 and Neurobasal medium (Gibco), with addition of 0.5% N2 supplement (Life Technologies), 0.5% ml MEM-NEAA (Gibco), 1% Glutamax (Gibco), 1% B27 supplement without vitamin A (Life Technologies), 0.1 μM of 2-mercaptoethanol (Millipore), 2.6 μg/ml insulin (Sigma Aldrich) supplemented with 10μM of SB431542 (STEMCELL), 2 μM of CHIR99021 (STEMCELL) and 0.5 μM of LDN-193189 (STEMCELL).

    Article Title: Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging.
    Article Snippet: Briefly, for hCS, 9000 iPSCs were first treated with 20 μM of ROCK inhibitor (S1049, Selleckchem) in neural induction medium containing 20% knockout serum replacement (10828028, Gibco), 1% GlutaMax (35050061, Gibco), 1% MEM-NEAA (11140035, Gibco), 0.1 mM of 2-mercaptoethanol (21985023, Gibco), and 1% penicillin/streptomycin (PS-B, Capricorn) in DMEM/F12 media.

    Article Title: Altered Protein Phosphorylation in a Novel Midbrain Organoid Model for Bipolar Disorder
    Article Snippet: Starting at day 14, media changes took place every other day using Midbrain 4th media Neurobasal medium (Gibco/Fisher Scientific), 1X B27 Supplement (Thermo Fisher), 1X MEM-NEAA (Gibco/Fisher Scientific), 1X GlutaMax (Thermo Fisher), 1X Penicillin/Streptomycin (Gibco/Fisher Scientific), 200 μM Ascorbic Acid (Sigma Aldrich), 500 μM cAMP (Sigma Aldrich), 20 ng/mL BDNF (Peprotech/Thermo Fisher), 20 ng/mL GDNF (Peprotech/Thermo Fisher), and 1 ng/mL TGF beta (Stemcell Technologies).

    Article Title: Maternal phthalates exposure promotes neural stem cell differentiation into phagocytic astrocytes and synapse engulfment via IRE1α/XBP1s pathway.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pHrodo Thermo Fisher Scientific Cat#P36600 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat#13778150 mTeSRTM Plus medium STEMCELL Technologies Cat#100-0276 Matrigel Corning Cat#356231 ReleSR STEMCELL Cat#100-0484 Y-27632 STEMCELL Technologies Cat#72308 knockout serum replacer Thermo Fisher Scientific Cat#17504044 MEM-NEAA Thermo Fisher Scientific Cat#11140050 dorsomorphine Tocris Cat#3093/10 A83-01 Tocris Cat#2939/10 b-mercaptoethanol Gibco Cat#21985023 N2 supplement Thermo Fisher Scientific Cat#17502048 Heparin Meilunbio Cat#MB1908 SB431542 Abcame Cat#ab120163 LDN193189 Sigma Cat#SML0559 Insulin Beyotime Cat#P3376-400IU MKC8866 Fosun Orinove N/A MBP MACKLIN Cat#131-70-4 Tamoxifen MedChemExpress Cat# HY-13757A Critical commercial assays Cell Count Kit-8 Vazyme Cat#A311-01 DAB kit Absin Bioscience Cat#ABS957 FD Rapid GolgiStainTM Kit FD NeuroTechnologies Cat#PK-401 Universal cDNA synthesis kit Vazyme Cat#R233-01 SYBR Green master mix Vazyme Cat#Q311-02 TRIzol reagent box Thermo Fisher Scientific Cat#15596018 Firefly & Renilla Luciferase Reporter Assay Kit Meilunbio Cat#MA0518 Fluo-3a.m.

    Article Title: Mechanical impact on neural stem cell lineage decisions in human brain organoids
    Article Snippet: ODM consisted of a 1:1 mix of DMEM/F12 + GlutaMAX-I (Thermo Fisher) and Neurobasal medium (Thermo Fisher), 0,5% N2 supplement (Thermo Fisher), 0.1 mM MEM-NEAA (Thermo Fisher), 100 U/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher), 1% B27 +/- vitamin A supplement (Thermo Fisher), 0.025% insulin (Sigma-Aldrich), 0.035% 2-mercaptoethanol (Sigma-Aldrich).

    Article Title: A stroke organoids-multiomics platform to study injury mechanism and drug response
    Article Snippet: Stroke is one of the top causes of death and disability worldwide, and its pathogenesis and mechanism have not been fully elucidated.. Several agents have shown protective effects against stroke in animal models; however, few studies have shown obvious effects in clinical practice.. This might be due to differences in brain structure and physiological function between humans and rodents.

    Article Title: Impaired Myelination in Multiple Sclerosis Organoids: p21 Links Oligodendrocyte Dysfunction to Disease Subtype
    Article Snippet: After neuroepithelium emergence (typically at ∼ day 9-10), embryoid bodies were embedded in 15 μL Matrigel droplets and cultured in 6 well plates containing c-organoid differentiation medium consisting of 1:1 DMEM-F12 and Neurobasal medium (Gibco), with addition of 0.5% N2 supplement (Life Technologies), 0.5% ml MEM-NEAA (Gibco), 1% Glutamax (Gibco), 1% B27 supplement without vitamin A (Life Technologies), 0.1 μM of 2-mercaptoethanol (Millipore), 2.6 μg/ml insulin (Sigma Aldrich) in static culture for 4 days.

    SYBR Green Assay:

    Article Title: Detection of low pre-existing humoral immunity against influenza virus H5N1 clade 2.3.4.4b in unexposed individuals
    Article Snippet: MDCK-SIAT1 (Merck, cat. no. 5071502) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma-Aldrich) containing 10% FBS (Sigma Aldrich), 1% Penicillin-Streptomycin (PS, Gibco), 1 mM L-Glutamine (Gibco) and 1% MEM-NEAA (Gibco).

    Article Title: Impaired Myelination in Multiple Sclerosis Organoids: p21 Links Oligodendrocyte Dysfunction to Disease Subtype
    Article Snippet: On day 3 to totality of the culture media was removed and replaced with a differentiation media consisting of 1:1 DMEM-F12 and Neurobasal medium (Gibco), with addition of 0.5% N2 supplement (Life Technologies), 0.5% ml MEM-NEAA (Gibco), 1% Glutamax (Gibco), 1% B27 supplement without vitamin A (Life Technologies), 0.1 μM of 2-mercaptoethanol (Millipore), 2.6 μg/ml insulin (Sigma Aldrich) supplemented with 10μM of SB431542 (STEMCELL), 2 μM of CHIR99021 (STEMCELL) and 0.5 μM of LDN-193189 (STEMCELL).

    Article Title: Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging.
    Article Snippet: Briefly, for hCS, 9000 iPSCs were first treated with 20 μM of ROCK inhibitor (S1049, Selleckchem) in neural induction medium containing 20% knockout serum replacement (10828028, Gibco), 1% GlutaMax (35050061, Gibco), 1% MEM-NEAA (11140035, Gibco), 0.1 mM of 2-mercaptoethanol (21985023, Gibco), and 1% penicillin/streptomycin (PS-B, Capricorn) in DMEM/F12 media.

    Article Title: Altered Protein Phosphorylation in a Novel Midbrain Organoid Model for Bipolar Disorder
    Article Snippet: Starting at day 14, media changes took place every other day using Midbrain 4th media Neurobasal medium (Gibco/Fisher Scientific), 1X B27 Supplement (Thermo Fisher), 1X MEM-NEAA (Gibco/Fisher Scientific), 1X GlutaMax (Thermo Fisher), 1X Penicillin/Streptomycin (Gibco/Fisher Scientific), 200 μM Ascorbic Acid (Sigma Aldrich), 500 μM cAMP (Sigma Aldrich), 20 ng/mL BDNF (Peprotech/Thermo Fisher), 20 ng/mL GDNF (Peprotech/Thermo Fisher), and 1 ng/mL TGF beta (Stemcell Technologies).

    Article Title: Maternal phthalates exposure promotes neural stem cell differentiation into phagocytic astrocytes and synapse engulfment via IRE1α/XBP1s pathway.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pHrodo Thermo Fisher Scientific Cat#P36600 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat#13778150 mTeSRTM Plus medium STEMCELL Technologies Cat#100-0276 Matrigel Corning Cat#356231 ReleSR STEMCELL Cat#100-0484 Y-27632 STEMCELL Technologies Cat#72308 knockout serum replacer Thermo Fisher Scientific Cat#17504044 MEM-NEAA Thermo Fisher Scientific Cat#11140050 dorsomorphine Tocris Cat#3093/10 A83-01 Tocris Cat#2939/10 b-mercaptoethanol Gibco Cat#21985023 N2 supplement Thermo Fisher Scientific Cat#17502048 Heparin Meilunbio Cat#MB1908 SB431542 Abcame Cat#ab120163 LDN193189 Sigma Cat#SML0559 Insulin Beyotime Cat#P3376-400IU MKC8866 Fosun Orinove N/A MBP MACKLIN Cat#131-70-4 Tamoxifen MedChemExpress Cat# HY-13757A Critical commercial assays Cell Count Kit-8 Vazyme Cat#A311-01 DAB kit Absin Bioscience Cat#ABS957 FD Rapid GolgiStainTM Kit FD NeuroTechnologies Cat#PK-401 Universal cDNA synthesis kit Vazyme Cat#R233-01 SYBR Green master mix Vazyme Cat#Q311-02 TRIzol reagent box Thermo Fisher Scientific Cat#15596018 Firefly & Renilla Luciferase Reporter Assay Kit Meilunbio Cat#MA0518 Fluo-3a.m.

    Article Title: Mechanical impact on neural stem cell lineage decisions in human brain organoids
    Article Snippet: ODM consisted of a 1:1 mix of DMEM/F12 + GlutaMAX-I (Thermo Fisher) and Neurobasal medium (Thermo Fisher), 0,5% N2 supplement (Thermo Fisher), 0.1 mM MEM-NEAA (Thermo Fisher), 100 U/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher), 1% B27 +/- vitamin A supplement (Thermo Fisher), 0.025% insulin (Sigma-Aldrich), 0.035% 2-mercaptoethanol (Sigma-Aldrich).

    Article Title: A stroke organoids-multiomics platform to study injury mechanism and drug response
    Article Snippet: Stroke is one of the top causes of death and disability worldwide, and its pathogenesis and mechanism have not been fully elucidated.. Several agents have shown protective effects against stroke in animal models; however, few studies have shown obvious effects in clinical practice.. This might be due to differences in brain structure and physiological function between humans and rodents.

    Article Title: Impaired Myelination in Multiple Sclerosis Organoids: p21 Links Oligodendrocyte Dysfunction to Disease Subtype
    Article Snippet: After neuroepithelium emergence (typically at ∼ day 9-10), embryoid bodies were embedded in 15 μL Matrigel droplets and cultured in 6 well plates containing c-organoid differentiation medium consisting of 1:1 DMEM-F12 and Neurobasal medium (Gibco), with addition of 0.5% N2 supplement (Life Technologies), 0.5% ml MEM-NEAA (Gibco), 1% Glutamax (Gibco), 1% B27 supplement without vitamin A (Life Technologies), 0.1 μM of 2-mercaptoethanol (Millipore), 2.6 μg/ml insulin (Sigma Aldrich) in static culture for 4 days.

    Luciferase:

    Article Title: Detection of low pre-existing humoral immunity against influenza virus H5N1 clade 2.3.4.4b in unexposed individuals
    Article Snippet: MDCK-SIAT1 (Merck, cat. no. 5071502) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma-Aldrich) containing 10% FBS (Sigma Aldrich), 1% Penicillin-Streptomycin (PS, Gibco), 1 mM L-Glutamine (Gibco) and 1% MEM-NEAA (Gibco).

    Article Title: Impaired Myelination in Multiple Sclerosis Organoids: p21 Links Oligodendrocyte Dysfunction to Disease Subtype
    Article Snippet: On day 3 to totality of the culture media was removed and replaced with a differentiation media consisting of 1:1 DMEM-F12 and Neurobasal medium (Gibco), with addition of 0.5% N2 supplement (Life Technologies), 0.5% ml MEM-NEAA (Gibco), 1% Glutamax (Gibco), 1% B27 supplement without vitamin A (Life Technologies), 0.1 μM of 2-mercaptoethanol (Millipore), 2.6 μg/ml insulin (Sigma Aldrich) supplemented with 10μM of SB431542 (STEMCELL), 2 μM of CHIR99021 (STEMCELL) and 0.5 μM of LDN-193189 (STEMCELL).

    Article Title: Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging.
    Article Snippet: Briefly, for hCS, 9000 iPSCs were first treated with 20 μM of ROCK inhibitor (S1049, Selleckchem) in neural induction medium containing 20% knockout serum replacement (10828028, Gibco), 1% GlutaMax (35050061, Gibco), 1% MEM-NEAA (11140035, Gibco), 0.1 mM of 2-mercaptoethanol (21985023, Gibco), and 1% penicillin/streptomycin (PS-B, Capricorn) in DMEM/F12 media.

    Article Title: Altered Protein Phosphorylation in a Novel Midbrain Organoid Model for Bipolar Disorder
    Article Snippet: Starting at day 14, media changes took place every other day using Midbrain 4th media Neurobasal medium (Gibco/Fisher Scientific), 1X B27 Supplement (Thermo Fisher), 1X MEM-NEAA (Gibco/Fisher Scientific), 1X GlutaMax (Thermo Fisher), 1X Penicillin/Streptomycin (Gibco/Fisher Scientific), 200 μM Ascorbic Acid (Sigma Aldrich), 500 μM cAMP (Sigma Aldrich), 20 ng/mL BDNF (Peprotech/Thermo Fisher), 20 ng/mL GDNF (Peprotech/Thermo Fisher), and 1 ng/mL TGF beta (Stemcell Technologies).

    Article Title: Maternal phthalates exposure promotes neural stem cell differentiation into phagocytic astrocytes and synapse engulfment via IRE1α/XBP1s pathway.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pHrodo Thermo Fisher Scientific Cat#P36600 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat#13778150 mTeSRTM Plus medium STEMCELL Technologies Cat#100-0276 Matrigel Corning Cat#356231 ReleSR STEMCELL Cat#100-0484 Y-27632 STEMCELL Technologies Cat#72308 knockout serum replacer Thermo Fisher Scientific Cat#17504044 MEM-NEAA Thermo Fisher Scientific Cat#11140050 dorsomorphine Tocris Cat#3093/10 A83-01 Tocris Cat#2939/10 b-mercaptoethanol Gibco Cat#21985023 N2 supplement Thermo Fisher Scientific Cat#17502048 Heparin Meilunbio Cat#MB1908 SB431542 Abcame Cat#ab120163 LDN193189 Sigma Cat#SML0559 Insulin Beyotime Cat#P3376-400IU MKC8866 Fosun Orinove N/A MBP MACKLIN Cat#131-70-4 Tamoxifen MedChemExpress Cat# HY-13757A Critical commercial assays Cell Count Kit-8 Vazyme Cat#A311-01 DAB kit Absin Bioscience Cat#ABS957 FD Rapid GolgiStainTM Kit FD NeuroTechnologies Cat#PK-401 Universal cDNA synthesis kit Vazyme Cat#R233-01 SYBR Green master mix Vazyme Cat#Q311-02 TRIzol reagent box Thermo Fisher Scientific Cat#15596018 Firefly & Renilla Luciferase Reporter Assay Kit Meilunbio Cat#MA0518 Fluo-3a.m.

    Article Title: Mechanical impact on neural stem cell lineage decisions in human brain organoids
    Article Snippet: ODM consisted of a 1:1 mix of DMEM/F12 + GlutaMAX-I (Thermo Fisher) and Neurobasal medium (Thermo Fisher), 0,5% N2 supplement (Thermo Fisher), 0.1 mM MEM-NEAA (Thermo Fisher), 100 U/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher), 1% B27 +/- vitamin A supplement (Thermo Fisher), 0.025% insulin (Sigma-Aldrich), 0.035% 2-mercaptoethanol (Sigma-Aldrich).

    Article Title: A stroke organoids-multiomics platform to study injury mechanism and drug response
    Article Snippet: Stroke is one of the top causes of death and disability worldwide, and its pathogenesis and mechanism have not been fully elucidated.. Several agents have shown protective effects against stroke in animal models; however, few studies have shown obvious effects in clinical practice.. This might be due to differences in brain structure and physiological function between humans and rodents.

    Article Title: Impaired Myelination in Multiple Sclerosis Organoids: p21 Links Oligodendrocyte Dysfunction to Disease Subtype
    Article Snippet: After neuroepithelium emergence (typically at ∼ day 9-10), embryoid bodies were embedded in 15 μL Matrigel droplets and cultured in 6 well plates containing c-organoid differentiation medium consisting of 1:1 DMEM-F12 and Neurobasal medium (Gibco), with addition of 0.5% N2 supplement (Life Technologies), 0.5% ml MEM-NEAA (Gibco), 1% Glutamax (Gibco), 1% B27 supplement without vitamin A (Life Technologies), 0.1 μM of 2-mercaptoethanol (Millipore), 2.6 μg/ml insulin (Sigma Aldrich) in static culture for 4 days.

    Reporter Assay:

    Article Title: Detection of low pre-existing humoral immunity against influenza virus H5N1 clade 2.3.4.4b in unexposed individuals
    Article Snippet: MDCK-SIAT1 (Merck, cat. no. 5071502) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma-Aldrich) containing 10% FBS (Sigma Aldrich), 1% Penicillin-Streptomycin (PS, Gibco), 1 mM L-Glutamine (Gibco) and 1% MEM-NEAA (Gibco).

    Article Title: Impaired Myelination in Multiple Sclerosis Organoids: p21 Links Oligodendrocyte Dysfunction to Disease Subtype
    Article Snippet: On day 3 to totality of the culture media was removed and replaced with a differentiation media consisting of 1:1 DMEM-F12 and Neurobasal medium (Gibco), with addition of 0.5% N2 supplement (Life Technologies), 0.5% ml MEM-NEAA (Gibco), 1% Glutamax (Gibco), 1% B27 supplement without vitamin A (Life Technologies), 0.1 μM of 2-mercaptoethanol (Millipore), 2.6 μg/ml insulin (Sigma Aldrich) supplemented with 10μM of SB431542 (STEMCELL), 2 μM of CHIR99021 (STEMCELL) and 0.5 μM of LDN-193189 (STEMCELL).

    Article Title: Long-term tracking of neural and oligodendroglial development in large-scale human cerebral organoids by noninvasive volumetric imaging.
    Article Snippet: Briefly, for hCS, 9000 iPSCs were first treated with 20 μM of ROCK inhibitor (S1049, Selleckchem) in neural induction medium containing 20% knockout serum replacement (10828028, Gibco), 1% GlutaMax (35050061, Gibco), 1% MEM-NEAA (11140035, Gibco), 0.1 mM of 2-mercaptoethanol (21985023, Gibco), and 1% penicillin/streptomycin (PS-B, Capricorn) in DMEM/F12 media.

    Article Title: Altered Protein Phosphorylation in a Novel Midbrain Organoid Model for Bipolar Disorder
    Article Snippet: Starting at day 14, media changes took place every other day using Midbrain 4th media Neurobasal medium (Gibco/Fisher Scientific), 1X B27 Supplement (Thermo Fisher), 1X MEM-NEAA (Gibco/Fisher Scientific), 1X GlutaMax (Thermo Fisher), 1X Penicillin/Streptomycin (Gibco/Fisher Scientific), 200 μM Ascorbic Acid (Sigma Aldrich), 500 μM cAMP (Sigma Aldrich), 20 ng/mL BDNF (Peprotech/Thermo Fisher), 20 ng/mL GDNF (Peprotech/Thermo Fisher), and 1 ng/mL TGF beta (Stemcell Technologies).

    Article Title: Maternal phthalates exposure promotes neural stem cell differentiation into phagocytic astrocytes and synapse engulfment via IRE1α/XBP1s pathway.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pHrodo Thermo Fisher Scientific Cat#P36600 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat#13778150 mTeSRTM Plus medium STEMCELL Technologies Cat#100-0276 Matrigel Corning Cat#356231 ReleSR STEMCELL Cat#100-0484 Y-27632 STEMCELL Technologies Cat#72308 knockout serum replacer Thermo Fisher Scientific Cat#17504044 MEM-NEAA Thermo Fisher Scientific Cat#11140050 dorsomorphine Tocris Cat#3093/10 A83-01 Tocris Cat#2939/10 b-mercaptoethanol Gibco Cat#21985023 N2 supplement Thermo Fisher Scientific Cat#17502048 Heparin Meilunbio Cat#MB1908 SB431542 Abcame Cat#ab120163 LDN193189 Sigma Cat#SML0559 Insulin Beyotime Cat#P3376-400IU MKC8866 Fosun Orinove N/A MBP MACKLIN Cat#131-70-4 Tamoxifen MedChemExpress Cat# HY-13757A Critical commercial assays Cell Count Kit-8 Vazyme Cat#A311-01 DAB kit Absin Bioscience Cat#ABS957 FD Rapid GolgiStainTM Kit FD NeuroTechnologies Cat#PK-401 Universal cDNA synthesis kit Vazyme Cat#R233-01 SYBR Green master mix Vazyme Cat#Q311-02 TRIzol reagent box Thermo Fisher Scientific Cat#15596018 Firefly & Renilla Luciferase Reporter Assay Kit Meilunbio Cat#MA0518 Fluo-3a.m.

    Article Title: Mechanical impact on neural stem cell lineage decisions in human brain organoids
    Article Snippet: ODM consisted of a 1:1 mix of DMEM/F12 + GlutaMAX-I (Thermo Fisher) and Neurobasal medium (Thermo Fisher), 0,5% N2 supplement (Thermo Fisher), 0.1 mM MEM-NEAA (Thermo Fisher), 100 U/ml penicillin and 100 μg/ml streptomycin (Thermo Fisher), 1% B27 +/- vitamin A supplement (Thermo Fisher), 0.025% insulin (Sigma-Aldrich), 0.035% 2-mercaptoethanol (Sigma-Aldrich).

    Article Title: A stroke organoids-multiomics platform to study injury mechanism and drug response
    Article Snippet: Stroke is one of the top causes of death and disability worldwide, and its pathogenesis and mechanism have not been fully elucidated.. Several agents have shown protective effects against stroke in animal models; however, few studies have shown obvious effects in clinical practice.. This might be due to differences in brain structure and physiological function between humans and rodents.

    Article Title: Impaired Myelination in Multiple Sclerosis Organoids: p21 Links Oligodendrocyte Dysfunction to Disease Subtype
    Article Snippet: After neuroepithelium emergence (typically at ∼ day 9-10), embryoid bodies were embedded in 15 μL Matrigel droplets and cultured in 6 well plates containing c-organoid differentiation medium consisting of 1:1 DMEM-F12 and Neurobasal medium (Gibco), with addition of 0.5% N2 supplement (Life Technologies), 0.5% ml MEM-NEAA (Gibco), 1% Glutamax (Gibco), 1% B27 supplement without vitamin A (Life Technologies), 0.1 μM of 2-mercaptoethanol (Millipore), 2.6 μg/ml insulin (Sigma Aldrich) in static culture for 4 days.



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